Original Article

Irrigating Solutions lmpacts on Joint Cartilage. An Experimental In-vivo Study With Guinea Pigs

  • Nikola Azar
  • Cüneyt Mirzanlı

IMJ 2001;4(2):1-5


The establishment of this in vivo animal model, aims at studying the impacts onjoint cartilage tissue caused by; betadine, and several solutions replacing the synovial fluid during arthroscopy.

Material And Method:

60 knees of 30 Guinea pigs, were used for this research. Knees were divided into 5 groups. All groups were prone to below mentioned operations followed by synovial fluid aspiration. Group ı was used as the control group and only synovial fluid aspiratian was applied. 0.9% isotonic NaCl was injected to members of group 2, ringer lactate to 3, 5% glucose ringer to 4, and betadine to 5. Animals were sacrifıed at the postoperational 6th week.


The knee joint cartilage was macroscopically and microscopically evaluated. Microscopically detected damages are cartilage thinning, ulceration - erosion, fibrillation, fıssuring, cyst formation, osteophytic formation, proteoglycan loss, chondrocyt loss, chondrocyt cloning, subchondral bone uncovering and synovit and synovial proliferation. Signifıcant cartilage thinning, proteoglycan loss, chondrocyt loss and subchondral bone uncovering were observed in group 1. Cloning in chondrocyts was observed at substantial rates, which is a regeneration symptom. In spite of some differences, damage is in similar rates for group II, III and N (ll>lll>N).Substantial damage and high rate of synovitis and synovial proliferation were observed in group V.


As a consequence, joint fluid removal through punction, results in serious damage injoint. 0.9% isotonic saline solution, ringer lactate and 5% glucose ringer which are used in arthroscopy result in medium damage. Among these solutions, least damage is caused by 5% glucose ringer. Use of betadine ends up w ith very serious damage and synovitis and synovial proliferation.

Keywords: Arthroscopy, Irrigating solutions, cartilage damage